Effect of Rice Bran and Broken Rice on the Efficacy of Bacillus subtilis Antagonist Bacteria in Controlling Rigidoporus microporus, the Causing White Root Rot Disease on Para Rubber
Keywords:
Bacillus subtilis , Rigidoporus microporus, rice bran, broken riceAbstract
Rice bran and broken rice are the residues from the rice milling process, which contain the chemical composition. Using agricultural residues as a food source for microorganisms is also the benefit way to increase the value of the residues. The objective of this research was to study the effect of rice bran and broken rice on the growth of antagonistic bacteria Bacillus subtilis and Rigidoporus microporus as well as the efficacy of B. subtilis on the controlling R. microporus, which is the cause of white root disease of Para rubber. The result showed that antagonistic bacteria B. subtilis strain SM1 and LPDD3-2 in potato dextrose agar (PDA) supplemented with rice bran and with broken rice were able to grow more than 12 log CFU/ml, but the growth of R. microporus strain NK6 was decreased in PDA supplemented with rice bran. The antagonistic B. subtilis strain LPDD3-2 increased significantly (P<0.05)against mycelium growth of R. microporus on PDA supplemented with rice bran by dual culture test, especially on PDA supplemented with 3% rice bran had shown highest inhibition at 67.50 percent. Therefore, the bio-agent development of B. subtilis antagonistic bacteria for controlling R. microporus by using rice bran in culture medium of B. subtilis and as an ingredient in bio-agent is an approach that should be further studied.
References
Chanprathin, U. 2005. Diseases, Insect pests and Biological control. Bangkok: Department of Agriculture Press.
Chaurasia, B., Pandey, A., Palni, L.M.S., Trivedi, P., Kumar, B. and Colvin, N. 2005. Diffusible and volatile compounds produced by an antagonistic Bacillus subtilis strain cause structural deformations in pathogenic fungi in vitro. Microbiological Research 160: 75-81.
Daou, C. and Zhang, H. 2012. Effect of High Pressure, Autoclaving and Extrusion Treatments on Insoluble, Soluble and Total Dietary Fiber Contents of Defatted Rice Bran. Academic Food Journal 10: 6-13.
Demirci, T., Aktaş, K., Sozeri, D., Ozturk, H.I. and Akin, N. 2017. Rice bran improve probiotic viability in yoghurt and provide added antioxidative benefits. Journal of Functional Foods 36: 396-403.
Demissie, Y., Humblot, C., Baxter, B., Nealon, N.J. and Ryan, E. 2020. Probiotic fermentation of rice bran with six genetically diverse strains effects nutrient and phytochemical composition; a non-targeted metabolomics approach. Current Developments in Nutrition 4: 1553. https://doi.org/10.1093/cdn/nzaa062_010
Frank, S.A. 2010. The trade-off between rate and yield in the design of microbial metabolism. Journal of Evolutionary Biology 23: 609-613.
Khedherab, S.B., Trabelsib, B.M. and Tounsia, S. 2021. Biological potential of Bacillus subtilis V26 for the control of Fusarium wilt and tuber dry rot on potato caused by Fusarium species and the promotion of plant growth. Biological Control 152: 104444.
Kilian, M., Steiner, U., Krebs, B., Junge, H., Schmiedeknecht, G. and Hain, R. 2004. FZB24® Bacillus subtilis – mode of action of a microbial agent enhancing plant vitality. Pflanzenschutz-Nachrichten Bayer 100: 72-193.
Liu, Y., Zhang, H., Yi, C., Quan, K. and Lin, B. 2021. Chemical composition, structure, physicochemical and functional properties of rice bran dietary fiber modified by cellulase treatment. Food Chemistry 342: 128352. https://doi.org/10.1016/j.foodchem.2020.128352
Moon, S.H. and Chang, H.C. 2021. Rice bran fermentation using Lactiplantibacillus plantarum EM as a starter and the potential of the fermented rice bran as a functional food. Foods 10: 978. https://doi.org/10.3390/foods10050978
Ongena, M. and Jacques, P. 2008. Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends in Microbiology 16: 115-125.
Parker, E.J. 2000. Signalling in plant disease resistance. In: Molecular Plant Pathology. (ed. M. Dickinson and J. Beynon). pp. 198-217. Sheffield Academic Press: Sheffield, U.K.
Pengnoo, A., Wiwatanapatapee, R., Chumthong, A., Rotniam, W. and Kanjanamaneesathian, M. 2005. Preliminary study on the effect of culture medium on the number and size of endospores of Bacillus megaterium. Silpakorn University Science and Technology Journal 5: 129-139.
Pholthaweechai, U. and Pengnoo, A. 2021. Effect of Nitrogen and Bacillus subtilis SM1 Strain on Controlling Rigidoporus microporus NK6 Strain the Cause of White Root Rot Disease In Vitro Testing. Songklanakarin Journal of Plant Science 8: 44-49.
Rabbee, M.F., Ali, M.S., Choi, J., Hwang, B.S., Jeong, S.C. and Baek, K. 2019. Bacillus velezensis: A valuable member of bioactive molecules with in plant microbiomes. Molecules 24: 1046-1059.
Sompong, S., Siebenhandl-Ehn, S., Linsberger-Martina, E. and Berghofera, E. 2011. Physicochemical and antioxidative properties of red and black rice varieties from Thailand, China and Sri Lanka. Food Chemistry 124: 132-140.
Sungtong, S. 2021. Antagonistic Bacteria for Controlling White Root Rot Disease of Rubber (Hevea brasilieansis). Degree of Master of Science in Soil Resource Management. Prince of Songkla University.
Sungtong, S., Pngnoo, A. and Boonyapipat, P. 2021. Efficacy of Bacillus spp. in Controlling Soil borne Pathogen Rigidoporus microporus under Control Conditions. Songklanakarin Journal of Plant Science 8: 34-43.
Torres M. J., Perez Brandan C., Sabate D. C., Petroselli G., Erra-Balsells R. and Audisio M. C. 2017. Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPRacCA1 on common bean Phaseolus vulgaris L. pathogens. Biological Control 105: 93-99.
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