Effect of Nitrogen and Bacillus subtilis SM1 Strain on Controlling Rigidoporus microporus NK6 Strain the Cause of White Root Rot Disease In Vitro Testing
Keywords:
Bacillus subtilis, Rigidoporus microporus, nitrogen, biological controlAbstract
Nitrogen is an essential macronutrient for plant function and soil microorganisms. The southern soils are highly developed with easy loss of nutrients, especially nitrogen. The continuous plantation of rubber (Hevea brasiliensis) in the same field and neglected proper fertilization result in insufficient nutrient intake, weak rubber plant, and white root disease of para rubber (Rigidoporus microporus). Bacillus subtilis has been reported as antagonistic to a variety of plant pathogens. The objective of this work was to study the effect of nitrogen and B. subtilis SM1 strain for inhibiting R. microporus NK6 strain on white root rot disease (R. microporus NK6 strain) in laboratory. Nitrogen concentrations of 0, 0.421, 0.842, 1.263 and 1.684 M with four replications were tested for their growth of B. subtilis strain SM1, R. microporus strain NK6 and suppressive ability of B. subtilis strain SM1 against R. microporus strain NK6. The results showed that B. subtilis strain SM1 was able to grow up to 1011 cfu/ml, but the growth of R. microporus strain NK6 was decreased. However, with nitrogen and B. subtilis strain SM1 combination has also inhibited the growth of R. microporus NK6 strain mycelium by more than 80 percent. There should be further studies in greenhouses conditions and on the effects of other nutrients on the disease control.
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