Efficacy of Bacillus spp. in Controlling Soilborne Pathogen Rigidoporus microporus under Control Conditions
Keywords:
Bacillus spp., Rigidoporus microporus, biological controlAbstract
Soilborne fungus, Rigidoporus microporus causing white root rot disease is one of the important problems in rubber producing countries including Thailand. To reduce the fungicide use, therefore, biological control is needed to control the disease. Bacillus species show broad-spectrum antimicrobial activity and have been widely used as agricultural biocontrol agents. This study aimed to screen Bacillus spp. which were effective in inhibiting R. microporus. Bacillus spp. of 44 isolates were tested for their suppressive ability against R. microporus using dual culture plate technique with four replications. The results indicated that isolates SM1 and LPDD3-2 of Bacillus spp. were effective in controlling R. microporus with the rates of 66.43 and 54.29 percent, respectively. Mycelial deformity of R. microporus which was observed by microscopy and SEM confirmed the pored and wrinkle mycelia when the bacteria attached on the surface. Isolates SM1 and LPDD3-2 were taken at concentrations of 104, 105 and 106 cfu/ml, to test for their efficacy against R. microporus. At the concentration of 106 cfu/ml, antagonisitic substances and volatile compounds of isolate SM1 inhibited mycelial growth of R. microporus with the rates of 66.43 and 54.29 percent, respectively.
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