Effect of Culture Media and Explant Types on Development of Indian Oak (Barringtonia acutangula L.) In Vitro

Y. Wiriyananont; P. Sae - Ung

Authors

Y. Wiriyananont Faculty of Science and Technology, Phranakhon Si Ayutthaya Rajabhat University, Phra Nakhon Si Ayutthaya, 13000
P. Sae - Ung Faculty of Science and Technology, Phranakhon Si Ayutthaya Rajabhat University, Phra Nakhon Si Ayutthaya, 13000

Keywords:

Barringtonia acutangula L., micropropagation, conservation

Abstract

Indian Oak (Barringtonia acutangula L.) is a local plant that is found in Thailand, commonly grown as an ornamental plant, and has medicinal properties. They will be destroyed by the cultivation of other crops instead. Therefore, this study aimed to propagate the Indian Oak under a sterile condition. The explants were collected through the sterilization process and cultured on MS medium (Murashige and Skoog, 1962) combined with 2 mg/l 2,4-D (2,4-dichorophenoxyacetic acid) and BA (6-Benzyladenine). After 8 weeks of culture, the explants began to develop. The embryo and young leaves develop into compact a callus, pale yellow in color and petal explant develop into a pale-yellow friable callus, while anther and shoot tip was not developed. When the shoot obtained from in vitro culture was cut and cultured on MS with 0-2.5 mg/l BA. The result showed that the total number of shoots at all concentrations of BA was not statistically different (P≥0.05). After culturing friable callus derived from young petals on MS or WPM (Woody Plant Medium) with 2 mg/l 2,4-D, there was no statistically significant difference in callus proliferation on both media (P≥0.05). These calluses can grow well and can be used as an initial cell to induce suspension cells for further producing secondary metabolites. From the results of this study, Indian Oak was conserved in vitro for further utilization.

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