Effect of Biochar Derived from Spent Mushroom Substrates (DOA Mushchar) for Improving the Production Efficiency of Mother Culture and Mother Spawn of Lingzhi Mushroom

Abstract

Lingzhi mushroom is the one of economically important medicinal mushrooms with high medical properties and is extensively used for medical benefits. However, in the production process of mother culture and mother spawn, some strains of lingzhi mushrooms have been suffered from slow growth mycelium and irregular colony growth. The objectives of this study were to improve and develop technology to produce mother culture and mother spawn of lingzhi mushroom. Three strains of lingzhi mushrooms (GA025 GA026 and GA027) were applied to PDA culture and sorghum media from biochar derived from spent mushroom substrates (DOA Mushchar) at different concentrations. The results showed that on PDA + DOA Mushchar 0.1%, GA025, and GA027 were the highest mycelium diameter growth at 89.18 and 89.68 mm respectively. GA026, PDA + DOA Mushchar 0.2% was the highest mycelium diameter growth at 90.00 mm. While sorghum mother spawn + DOA Mushchar 0.1%, GA025 and GA026 were the fastest mycelium total colonization at 8.1 and 7.05 days respectively. GA027, sorghum + DOA Mushchar 0.2% was the fastest mycelium total colonization at 7.50 days. Therefore, DOA Mushchar at 0.1% concentration is the lowest concentration recommended for application in the production of mother culture and mother spawn for serviceability and knowledge transfer to mushroom growers in the future.